Accel-NGS Adaptase® Module

Explore the Methylome at Single Cell Resolution

Swift Accel-NGS® Adaptase

The Accel-NGS Adaptase Module constructs NGS libraries from bisulfite-converted DNA from single cells. The Adaptase Module maximizes the recovery of  low concentrations of single stranded DNA and libraries consistently exhibit superior complexity with reduced composition bias to provide a more faithful representation of the methylome. Using the in-line barcoding option, cells can be mulitplexed to increase throughput and further reduce library prep costs.

Swift Adaptase video

The Adaptase Module can be used for many applications, such as cataloging cell populations within heterogeneous tissues, assessing normal tissue for regulation of cellular mechanisms (e.g., differentiation), gaining insight into epigenetic alterations in disease states, and exploring across species to identify evolutionary conservation of epigenomic regulation.

Features:

  • High mapping rate
  • >400 bp library insert size improves data output
  • Workflow uses a single random priming cycle

Benefits:

  • Greater data output per run and reduced sequencing cost
  • Scalable cell and library indexing options for high throughput sequencing
  • Easier low bias workflow

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Note: The 192 reactions kits are sold as a bundle of two 96 reaction kits.

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The Accel-NGS Adaptase Module requires commercially-available oligonucleotide primers, enzymes, and buffers (ordered separately) for complete functionality. Please see the Accel-NGS Adaptase Module for Single-Cell Methyl-Seq Library Preparation Protocol for a complete list of required third-party materials not supplied by Swift Biosciences.

For Customers Outside the United States: Please request a quote or select from our list of distributors.

KEY PUBLICATIONS
VIEW ARTICLES
INDEXING SELECTION GUIDE
DOWNLOAD PDF
WORKFLOW
VIEW DOCUMENT
PROFILING PEDIATRIC TUMORS WEBINAR
VIEW WEBINAR
PROTOCOLS, TOOLS & TECHNICAL NOTES
VIEW DOCUMENT

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