The Accel-NGS Adaptase Module constructs NGS libraries from bisulfite-converted, single-stranded DNA from single cells. Because bisulfite treatment results in lower quantity and quality DNA, the module is optimized to maximize the recovery of DNA containing uracil residues and low concentrations of AT-rich template. The resultant libraries consistently exhibit superior complexity with reduced composition bias to provide a more faithful representation of the methylome.
The Accel-NGS Adaptase Module is an excellent choice for single cell methylation applications. This approach incorporates a three-dimensional indexing strategy to generate 96 individual libraries that can be pooled and sequenced up to 384 individual cells in parallel. The published results demonstrated greater than two-fold increase in read mapping rate as compared to other methods and significantly improves the data output per run while reducing the sample sequencing cost.
It can be used for many applications, such as cataloging cell populations within heterogeneous tissues, assessing normal tissue for regulation of cellular mechanisms (e.g., differentiation), gaining insight into epigenetic alterations in disease states, and exploring across species to identify evolutionary conservation of epigenomic regulation.
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The Accel-NGS Adaptase Module requires commercially-available oligonucleotide primers, enzymes, and buffers (ordered separately) for complete functionality. Please see the Accel-NGS Adaptase Module for Single-Cell Methyl-Seq Library Preparation Protocol for a complete list of required third-party materials not supplied by Swift Biosciences.
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