Swift Applications

Swift products enable the most efficient and reproducible output from routine and challenging samples, for next-generation sequencing of DNA and RNA.

Epigenetics is a major determining factor in cellular differentiation, growth, disease progression and therapeutic response.  Along with environmental factors such as diet and stress, changes in the epigenome regulate gene expression and may have profound impacts on cancer and other complex diseases. However, very little is known about which genes are turned on or off, the role of epigenetics in heritability, or how these changes can lead to disease onset.

Swift offers innovative next-generation sequencing (NGS) library prep solutions for methylation sequencing (Methyl-Seq) and Chromatin IP sequencing (ChIP-Seq) to help you extract more genomic information from limited specimens.  Whether working with single cells or picogram-level inputs from small amounts of complex tissue, our sensitivity and robust technologies help you unlock challenging samples to make bigger, faster scientific breakthroughs.

As next-generation sequencing (NGS) costs decrease and bioinformatics analysis pipelines improve, the utility of whole-genome sequencing (WGS) is ever expanding. However, as WGS sample throughput increases, preparing high-quality sequenceable libraries from a wide range of sample types in a timely manner is a bottleneck impeding sequencing productivity.

Whether you are new to NGS or your capabilities already include high throughput automated WGS, our Accel-NGS® 2S DNA Library Kits can help you maximize your data output, giving you the best genome-wide coverage of any kit on the market, and reducing your sequencing costs, while with the Swift Amplicon Sample ID Panel you will confidently track and manage samples during your human genotyping studies. Additionally, Swift is adept at making high throughput custom genotyping panels for agriculture.

Circulating nucleic acids have emerged as important biomarkers for cancer diagnostics and the detection of various clinical conditions. Utilizing liquid biopsy as an alternative to invasive and at times inaccessible tumor biopsy may enable access to critical information about disease composition and progression. Translational genomics research is utilizing both circulating cell-free DNA (cfDNA) and circulating tumor DNA (ctDNA) to monitor disease based on specific tumor profiles. To access this valuable information, it is critical to use next-generation sequencing (NGS) to detect variants in low levels of cfDNA from blood samples.

Swift Biosciences’ advanced technology offers an array of pre-designed and customizable Accel-Amplicon™ and Swift Amplicon High Sensitivity (HS) Panels optimized for multiple sequencing platforms to enable a fast, comprehensive approach to targeted liquid biopsy sequencing.

Next-generation sequencing (NGS) of DNA extracted from metagenomic samples has enabled the identification and characterization of low abundance and unculturable bacteria within environmental samples. There are two main approaches to metagenomics sequencing; shotgun and targeted sequencing. Shotgun sequencing provides the most comprehensive snapshot of microbial diversity, although it is usually cost-prohibitive, and hindered by host DNA contamination, unlike targeted approaches, which are more cost-effective and not as affected by host contamination.

Swift offers competitive solutions for both types of approaches. Swift 2S Turbo DNA Library kits paired with the Swift Normalase™ Kits provide a fast, efficient and relatively cost-effective solution for metagenomics shotgun sequencing. While for targeted sequencing, the Swift Amplicon 16S +ITS Panel profiles complex metagenomic samples by targeting the entire16S rRNA (V1-V9) gene and ITS genes in a single primer pool, along with a simple two-hour workflow, ensuring sensitive and specific identification of a wide variety of microbes.

RNA sequencing (RNA-Seq) is a powerful tool with a multitude of applications, including whole-transcriptome profiling, the identification of novel transcripts, the detection of coding variants, the detection of fusion genes, and the identification of alternative splicing, among others.

Transcriptomic studies have the ability to provide a snapshot of gene expression and regulation within a particular tissue or cell type. For example, expression level studies have provided insight into the long-appreciated relationship between temperature and sex-determination in some reptiles, such as crocodiles and turtles. During embryo development, higher temperatures result in females hatching, lower temperatures result in males hatching, and intermediate temperatures result in a mix of both. Transcriptomic studies revealed that this determination is caused by changes in expression of the Sox9 gene. At lower temperatures, Sox9 has higher expressional levels whereas higher temperatures show repressed expression of Sox9. This expression impacts the amount of a transcription factor produced by Sox9, which then impacts a gene that plays a major role in sex determination.