Hybridization Capture Sequencing
Targeted sequencing is a cost-effective alternative to whole genome sequencing since higher depth of coverage for specific regions can be obtained from less sequencing. Whole exome sequencing utilizing hybridization capture-based methods remains a predominant process for discovery because entire protein coding regions of genes constitute the majority of known disease causing variants. Alternatively, smaller disease specific or custom-based panels provide increased sensitivity and more efficient data analysis by focusing on targeted regions most likely to contain variants of interest. In either case, an unbiased NGS library must be generated in order to maximize the potential sensitivity in the selected hybridization capture panel.
Swift Biosciences offers a solution to construct bias-free libraries for target enrichment utilizing hybridization-based capture technologies. The Accel-NGS® 2S Hyb DNA Library Kit produces libraries with exceptional complexity and uniform coverage from high quality gDNA, FFPE, and cfDNA samples, regardless of input, to improve confidence in data analysis throughout the target region. Libraries generated with the kit are compatible with a variety of commercially available hybridization capture technologies, including Agilent SureSelectXT and SureSelectXT2, NimbleGen™ SeqCap™ EZ, and IDT® xGen® Lockdown® Probes for whole exome sequencing, disease specific or custom-generated panels.
SWIFT PRODUCT LINES COMPATIBLE WITH HYBRIDIZATION CAPTURE SEQUENCING:
Accel-NGS 2S Hyb DNA Library Kit
The Swift advantage:
- Compatible with multiple capture technology suitable for a diverse set of panels.
- Highly efficient adapter ligation provides meaningful data from low input samples.
- Exceptional library complexity results in more unique molecules available for capture.
- No adapter titration or heat steps make the kit readily automatable.