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Accel-Amplicon™ BRCA1, BRCA2, and PALB2 Panel

Targeted Sequencing for Breast Cancer Genes

All-in-One Amplicon Solution

The Accel-Amplicon BRCA1, BRCA2, and PALB2 Panel offers comprehensive coverage of the entire coding sequence of the BRCA1 and BRCA2 genes, as well as coverage of all coding exons and 5’ and 3’ UTR regions of PALB2 (partner and localizer of BRCA2). Utilizing 302 amplicons with an average size of 149 bp, this panel generates targeted libraries compatible with Illumina® and Ion Torrent™ sequencing platforms. The unique design of the Accel-Amplicon BRCA1, BRCA2, and PALB2 Panel enables compatibility with FFPE and cfDNA samples. This product is a complete kit that includes all components necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters.

Features:
  • Single-tube assay
  • Ready-to-sequence libraries in 2 hours
  • Inputs as low as 10 ng
Benefits:
  • Average amplicon size of 149 bp for compatibility with FFPE and cfDNA
  • Limit of detection as low as 1-5% for somatic mutations
  • On-target specificity and coverage uniformity > 95%
  • Leverages the high fidelity performance of the Illumina platform
  • Complete library generation in a single kit

The Accel-Amplicon workflow uses a fast, single-tube approach consisting of a 90-minute target enrichment amplification step and a 10-minute adapter ligation step, yielding a 2-hour start-to-finish procedure.

amplicon-workflow-100416

The single-tube workflow includes two brief incubations to generate the multiplex amplicon targets and add a unique combination of Illumina-compatible indexed adapters, creating up to 96 uniquely-indexed libraries for multiplexing on a single sequencing run.

Decrease Input, Not Sensitivity

Like the Accel-Amplicon BRCA1 and BRCA2 Panel, the Accel-Amplicon BRCA1, BRCA2, and PALB2 Panel offers high sensitivity variant detection from input amounts from 10-25 ng. The kit utilizes Illumina-compatible dual-indexed adapter sequences and has been validated on the MiSeq® and HiSeq® platforms. Barcoded adaptors for sequencing on Ion Torrent platforms are also available (please inquire).

amplicon_brca1_2_palb2_specs

*As quantified by qPCR. Qubit® represents amplifiable DNA content more accurately than NanoDrop®, however is still not as accurate as the qPCR assay. For sample types with more consistent high quality DNA including whole blood, fresh frozen samples, and cultured cells, quantification by Qubit is a reliable indicator of amplifiable content.
Comprehensive Coverage of Coding Sequences of BRCA1

amplicon_brca1_comp-coverage

Coverage of all coding exons of the BRCA1 gene by Accel-Amplicon BRCA1 and BRCA2 Panel are represented in a Sashimi plot (IGV; Broad Institute).
Comprehensive Coverage of Coding Sequences of BRCA2

amplicon_brca2_comp-coverage

Coverage of all coding exons of the BRCA2 gene by Accel-Amplicon BRCA1 and BRCA2 Panel are represented in a Sashimi plot (IGV; Broad Institute).
Comprehensive Coverage of Coding Sequences of PALB2

Amplicon_BRCA PALB2_Comp Coverage

Coverage of all coding exons of the PALB2 gene by the Accel-Amplicon BRCA1, BRCA2, and PALB2 Panel are represented in a Sashimi plot (IGV; Broad Institute).

 Performance on Illumina Platform

The data below demonstrate the performance of the Accel-Amplicon BRCA1, BRCA2, and PALB2 Panel on a variety of sample types.

amplicon_table_brca1_2_palb2_input

The Accel-Amplicon BRCA1, BRCA2, and PALB2 Panel was used to prepare libraries from 10 ng inputs of high quality Coriell NA12878 gDNA and Horizon Diagnostics Quantitative Multiplex DNA Reference Standards HD701. Sequencing was performed using MiniSeq® Reagents.

Place Order

First, select a product and quantity:

Catalog No. Description Price QTY
AL-57048 Accel-Amplicon BRCA1, BRCA2, and PALB2 Panel (48 rxns) $3600.00

$3,600.00 Add to cart

And, add optional components:

Catalog No. Description Price QTY
90196  PEG NaCl Solution (96 rxns) $20.00

$20.00 Add to cart