Accel-Amplicon™ BRCA1 and BRCA2 Panel
All-in-One Amplicon Solution
The Accel-Amplicon BRCA1 and BRCA2 Panel offers comprehensive coverage of the entire coding sequence of BRCA1 and BRCA2 genes. Utilizing 246 amplicons with average size of 148 bp, this panel generates targeted libraries compatible with Illumina® and Ion Torrent sequencing platforms. The unique design of the Accel-Amplicon BRCA1 and BRCA2 Panel enables compatibility with FFPE and cfDNA samples. This product is a complete kit that includes all components necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters.
- Single-tube assay
- Ready-to-sequence libraries in 2 hours
- Inputs as low as 10 ng
- Average amplicon size of 148 bp for compatibility with FFPE and cfDNA
- Limit of detection as low as 1-5% for somatic mutations
- On-target specificity and coverage uniformity > 95%
- Leverages the high fidelity performance of the Illumina platform
- Complete library generation in a single kit
The Accel-Amplicon workflow uses a fast, single-tube approach consisting of a 90-minute target enrichment amplification step and a 10-minute adapter ligation step, yielding a 2-hour start-to-finish procedure.
The single-tube workflow includes two brief incubations to generate the multiplex amplicon targets and add a unique combination of Illumina-compatible indexed adapters, creating up to 96 uniquely-indexed libraries for multiplexing on a single sequencing run.
Decrease Input, Not Sensitivity
Like the Accel-Amplicon BRCA1, BRCA2, and PALB2 Panel, the Accel-Amplicon BRCA1 and BRCA2 Panel offers high sensitivity variant detection from input amounts from 10-25 ng. The kit utilizes Illumina-compatible dual-indexed adapter sequences and has been validated on the MiSeq® and MiniSeq® platforms. Barcoded adaptors for sequencing on Ion Torrent platforms are also available (please inquire).
*As quantified by qPCR. Qubit® represents amplifiable DNA content more accurately than NanoDrop®, however is still not as accurate as the qPCR assay. For sample types with more consistent high quality DNA including whole blood, fresh frozen samples, and cultured cells, quantification by Qubit is a reliable indicator of amplifiable content.
Comprehensive Coverage of Coding Sequences of BRCA1
Coverage of all coding exons of the BRCA1 gene by Accel-Amplicon BRCA1 and BRCA2 Panel are represented in a Sashimi plot (IGV; Broad Institute).
Comprehensive Coverage of Coding Sequences of BRCA2
Coverage of all coding exons of the BRCA2 gene by Accel-Amplicon BRCA1 and BRCA2 Panel are represented in a Sashimi plot (IGV; Broad Institute).
Performance on the Illumina Platform
The data below demonstrate the performance of the Accel-Amplicon BRCA1 and BRCA2 Panel on a variety of sample types.
The Accel-Amplicon BRCA1 and BRCA2 Panel was used to prepare libraries from 10 ng inputs of high quality Coriell (NA12878, NA24143,NA24695, and NA19240) gDNA, Horizon Diagnostics Quantitative Multiplex DNA Reference Standards HD701 and FFPE samples subjected to 6, 24, or 48 hours of fixation. Sequencing was performed using MiniSeq® Reagents.
Variant Calling by Accel-Amplicon BRCA1 and BRCA2 Panel
The Accel-Amplicon BRCA1 and BRCA2 Panel consistently detected validated variants at the expected allele frequency (AF) in replicates from 10 ng of the Horizon Diagnostics Quantitative Multiplex DNA Reference Standards HD701 and Coriell (NA14684, NA14805 and NA14788). The variants were called by GATK HaplotypeCaller (Broad Institute).
Protocols and Tools
- Accel-Amplicon Panels
- Quantification and Quality Assessment of Human DNA Samples
- Accel-Amplicon Master Mixing Volume Calculator
Contact Tech Support for sequencing data files (FASTQ, BAM, VCF, and BED) for Accel-Amplicon Panels.
First, select a product and quantity:
|AL-52048||Accel-Amplicon BRCA1 and BRCA2 Panel (48 rxns)||$3600.00|
And, add optional components:
|90196||PEG NaCl Solution (96 rxns)||$20.00|