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Accel-NGS® 1S Plus DNA Library Kit

DNA Library Preparation of Difficult Samples on Illumina® NGS Platforms

Rescue Valuable Sequencing Data from Precious Samples

The Accel-NGS 1S Plus DNA Library Kit enables users to make libraries from degraded and damaged DNA because innovative Swift technology makes library molecules from single-stranded DNA, as well as double-stranded DNA, in a single sample. This technology is the best choice for users needing to sequence difficult-to-process samples which cannot be sequenced by other methods; thus, allowing you to expand your research by processing damaged and degraded samples.

Supported DNA sequencing applications for the Accel-NGS 1S Plus Kit include metagenomics and ChIP-Seq. The Accel-NGS 1S Plus Kit is also compatible with FFPE, ancient DNA, and other samples containing single-stranded DNA.

Features:

  • Supports inputs as low as 10 pg
  • High fidelity polymerase
  • Adapts DNA > 40 bp long
  • Simple, 2-hour protocol

Benefits:

  • Reduce concerns of proper quantification
  • Minimize sequence bias from PCR
  • Capture previously unattainable sequences
  • Process more samples per day

16-1280_1s-plus-workflow-web

Note: clean-up beads are not provided with the kit. We recommend the SPRIselect™ Reagent Kit from Beckman Coulter.

Performance on the Illumina Platform

The data below demonstrate the performance of the Accel-NGS 1S Plus DNA Library Kit on ssDNA phages and difficult-to-process microbial samples.
Accurate Detection of Both ssDNA and dsDNA Phage

AP Metag chart 1S Plus detection of ssDNA and dsDNA Phage

The Accel-NGS 1S Plus DNA Library Kit was used to prepare and sequence three artificial viromes containing different proportions of the ssDNA phage PhiX174 and M13 mixed with dsDNA phage. In all cases, the proportions were preserved when sequenced with the Accel-NGS 1S Plus Kit without any prior whole genome amplification for detection of ssDNA phage.
DNA Extraction and Sequencing of the Hard to Extract Microbe, Facklamia sp.HGF4

Prod_1S Plus_Table_Extract Method

Prod_1S Plus_Table_Facklamia sp

AP_Metag_1SPlus-DNAExtraction

DNA extraction by NaOH boiling produced higher DNA yields from Facklamia sp. than bead beating, and in less time. Sequencing of the NaOH extracted DNA produced a high quality de novo assembled genome sequence that was indistinguishable from that produced from bead beating extracted DNA.

The Accel-NGS 1S Plus technology uses a simultaneous end repair, tailing, and adapter ligation reaction called Adaptase™ to add an adapter oligonucleotide onto the 3′ end of single-stranded DNA in a template-independent manner. The 5′ adapter ligation is then facilitated by priming off this 3′ adapter, extending through the insert, and creating a compatible end for the 5′ ligation. This technology is very unique in the NGS library market and also forms the basis for Swift’s Accel-NGS Methyl-Seq DNA Library Kit and Accel-NGS DNA Library Kit for Ion Torrent™.

In some cases, with extremely degraded DNA that has been heavily nicked or denatured as a result of decrosslinking or other high temperature steps, this kit can produce the highest recovery and conversion rates of input DNA. However, for standard FFPE samples, we recommend using the Accel-NGS 2S Plus DNA Library Kit to achieve a 2-3 fold increase in yields versus competing kits and is usually superior to that obtained with the Accel-NGS 1S Plus DNA Library Kit.

Yes. Many users are attracted to this technology because it can convert the short, single-stranded fragments common in ancient DNA into NGS library molecules. Please contact us at Tech Support for recommended modifications to the standard protocol.

No. The polymerase used in the 1S Plus kit does not exhibit uracil tolerance. Therefore, for methyl-seq and other applications requiring tolerance to uracil-containing sequences, we recommend the Accel-NGS Methyl-Seq DNA Library Kit.

Yes. First you must isolate the RNA and remove rRNA by ribodepletion or enrich eukaryotic mRNA by polyA enrichment, followed by fragmenting the mRNA and determining its size distribution. First-strand cDNA should then be generated by an RT reaction, purified and quantified. From the purified first-strand cDNA, you may proceed to Accel-NGS 1S Plus library preparation. Please contact us at Tech Support for further recommendations.

In most cases there is no effect. Please view our technical note titled Accel-NGS 1S Plus and Methyl-Seq: Tail Trimming for Better Data for more details on the tail and how to trim if necessary.

Place Order

First, select a product and quantity:

Catalog No. Description Price QTY
10024 Accel-NGS 1S Plus DNA Library Kit (24 rxns) $960.00

$960.00 Add to cart

10096 Accel-NGS 1S Plus DNA Library Kit (96 rxns) $3450.00

$3,450.00 Add to cart

Next, an Indexing Kit is required for complete functionality. Please select from the following:

Catalog No. Description Price QTY
16024  1S Plus Set A Indexing Kit (12 indices, 24 rxns) $120.00

$120.00 Add to cart

18096  1S Plus Dual Indexing Kit (96 unique combinations) $480.00

$480.00 Add to cart