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Accel-NGS® DNA Library Kit for Ion Torrent™

DNA Library Preparation for NGS on Ion Torrent Platforms

Save Time with a Simple, 2-hour Prep for Ion Torrent

Innovative Swift technology improves sample prep for NGS by expediting the process and delivering higher quality data. The Accel-NGS DNA Library Kit designed for the Ion Torrent platform is the only commercially-available kit capable of producing PCR-free libraries with as little as 5 ng of input DNA. This library kit is compatible with the following instruments: Ion Proton System, Ion PGM System, and Ion S5 System. Amplicon sequencing is the primary supported DNA sequencing application for the Accel-NGS DNA Library Kit for Ion Torrent. The kit is also compatible with nicked DNA, ChIP DNA, FFPE samples, cDNA, and other samples containing single-stranded DNA.

Features:

  • Fast 75 minute start-to-finish protocol
  • Easy streamlined 3-step protocol
  • Low 5 ng DNA input requirements

Benefits:

  • Reduces adapter dimer formation to maximize sequencing output
  • PCR-free to minimize base composition bias and fidelity issues
  • Compatible with nicked, damaged, and denatured samples

Accel-NGS technology uses a unique combination of molecular biology reagents to produce a protocol that is streamlined and fast (75 minutes start-to-finish).

Two of the steps are bead-based separations that eliminate the need for time-consuming, electrophoretic gel-based size selection. Each step is color coded to make the protocol easy to follow. The simplified Accel-NGS workflow can be readily automated.

Total Turnaround Time Comparison Versus Other Kits

Ion Torrent_Kit Comparison

Performance on the Ion Torrent PGM

Data below demonstrates performance of the Accel-NGS DNA Library Kit for Ion Torrent on the PGM system. Data comparing the Accel-NGS DNA kit with other commercially available DNA library kits are also presented.

PCR-Free Libraries Prepared from 5 ng DNA

E. coli genomic DNA samples were fragmented to an average size of 260 (Figure 1a) or 180 bp (Figure 1b) using standard sonication methods. Libraries were then size-selected using SPRI beads. Accel-NGS DNA Libraries for Ion Torrent were sequenced on Ion 316 chips with either 500 (1a) or 260 (1b) flows depending on desired read length.

Ion Torrent_100 bp_200 bp histograms

Read length histograms from 200bp (Figure 1a) and 100bp (Figure 1b) sequencing chemistries.

Prod_Ion Torrent_Table_Seq Run_100 AND 200

Summary of sequencing run on 100bp and 200bp chemistries.

Conclusion. Accel-NGS DNA Library Kit for Ion Torrent produces sequenceable libraries PCR-free from 5 ng of input DNA. Similar performance has been obtained with input quantities ranging from 5 ng up to 8 μg.

PCR-Free versus Competitive Kit

Samples of E. coli genomic DNA (10 ng) were fragmented to an average size of 260 bp using standard sonication methods. Swift Biosciences and Company N libraries were prepared per their respective published protocols. Libraries were then size selected using SPRI beads and sequenced on Ion 316 chips with 500 flows for 200 bp chemistry.

Ion Torrent_histogram_Comp N

Read length histograms of Swift Biosciences versus Company N.

Prod_Ion Torrent_Table_Seq Run_Comp N

Summary of sequencing run of Swift Biosciences versus Company N.

Conclusion. The majority of Company N kit results were adapter dimers. Accel-NGS DNA Library Kit produces sequenceable libraries from low input without PCR, while minimizing the formation of adapter dimers to maximize sequencing output.

Great Results in About Half the Time Versus Leading Kit

1 μg samples of E. coli genomic DNA were fragmented to an average size of 260 bp using standard sonication methods. Swift Biosciences and Company L libraries were prepared per their published protocols. The Swift Biosciences library was completed in 75 minutes while Company L required 160 minutes. Both libraries were size-selected on a Pippin Prep instrument and libraries were sequenced on Ion 316 chips with 500 flows for 200 bp chemistry.

Ion Torrent_histogram_Comp L

Read length histograms of Swift Biosciences versus Company L.

Prod_Ion Torrent_Table_Seq Run_Comp L

Summary of sequencing run of Swift Biosciences versus Company L.

Conclusion. Accel-NGS DNA Library Kit achieves comparable performance to the leading competitive kit in about half the time.

Validation on the Ion PGM and Proton

Data below demonstrates performance of the Accel-NGS DNA Library Kit for Ion Torrent with microbial genomes of varying base composition that were prepared and sequenced on the Ion Torrent PGM and Proton systems by an independent third party. Data comparing the Accel-NGS DNA Library Kit for Ion Torrent with the leading commercially available Ion DNA library kit is also presented.

Even Coverage from 1 ng to 100 ng Input

1 ng to 100 ng of S. aureus genomic DNA (33% GC) was prepared using the Accel-NGS DNA Library Kit for Ion Torrent PCR-free protocol. The top figure shows the cumulative fraction of genome covered vs. depth of coverage, with a theoretical perfect result (red) when the entire genome is at normalized depth. The bottom figure shows GC content of each read vs. theoretical in silico distribution and coverage.

Ion Torrent_Even Cov_1 to 100 ng

Ion Torrent_Even Cov_1 to 100 ng_mapped reads

Conclusion: The Accel-NGS DNA Library Kit for Ion Torrent produces libraries from an AT-rich genome with near-theoretical coverage from 100 ng down to 1 ng of input DNA.

Balanced Composition Genome Performance

S. pullorum genomic DNA (52% GC) was prepared using the Accel-NGS DNA Library Kit and Competitor L’s kit using their respective protocols. Similar results were obtained with E. coli, 51% GC (not shown). The top figure shows cumulative fraction of genome covered vs. depth of coverage, with theoretical perfect result (red) when the entire genome is at normalized depth. The bottom figure shows GC content of each read vs. theoretical in silico distribution and coverage.

Ion Torrent_Genome Cov_cumulative

Ion Torrent_Genome Cov_Mapped Reads

Conclusion: The Accel-NGS DNA Library Kit produces libraries from a balanced base composition genome with coverage comparable to Competitor L’s kit on both the PGM and Proton platforms.

Outperforms Leading Ion Torrent Library Prep Kit for GC-rich Genomes

B. pertussis genomic DNA (68% GC) was prepared using the Accel-NGS DNA Library Kit and Competitor L’s kit. The top figure shows cumulative fraction of genome covered vs. depth of coverage, with theoretical perfect result (red) when the entire genome is at normalized depth. The bottom figure shows GC content of each read vs. theoretical in silico distribution and coverage.

Ion Torrent_GC rich Genomes

Ion Torrent_GC rich Genomes_Mapped Reads

Conclusion: The Accel-NGS DNA Library Kit produces libraries from a GC-rich genome with coverage closer to the theoretical perfect result vs. Competitor L’s kit on the Ion Proton.

Outperforms the Leading Ion Torrent Library Prep Kit for AT-rich Genomes

P. falciparum genomic DNA (19% GC) was prepared using the protocol from Competitor L’s kit, which required PCR . Additional genomic DNA was prepared using the Accel-NGS DNA Library Kit for Ion Torrent PCR-free protocol. The top figure shows the cumulative fraction of genome covered vs. depth of coverage, with the theoretical perfect result (red) when the entire genome is at normalized depth. The bottom figure shows GC content of each read vs. theoretical in silico distribution and coverage. Results demonstrated that 5% of the genome was missing in Competitor L’s library prep with PCR while < 2% of the genome was missing in Swift Biosciences library prep without PCR.

Ion Torrent_AT rich Genomes

Ion Torrent_AT rich Genomes_Mapped Reads

Conclusion: The Accel-NGS DNA Library Kit produces libraries from AT-rich genomes with coverage closer to the theoretical perfect result vs. Competitor L’s kit on the Ion Proton.

Place Order

First, select a product and quantity:

Catalog No. Description Price QTY
11010 Accel-NGS DNA Library Kit for Ion Torrent (10 rxns) $300.00

$300.00 Add to cart

11050 Accel-NGS DNA Library Kit for Ion Torrent (50 rxns) $1250.00

$1,250.00 Add to cart

Next, an Indexing Kit is required for complete functionality. Please select from the following:

Catalog No. Description Price QTY
16110  Ion Set A Barcoding Kit (10 barcodes, 10 rxns) $50.00

$50.00 Add to cart

16150  Ion Set A Barcoding Kit (10 barcodes, 50 rxns) $250.00

$250.00 Add to cart

16210  Ion Set B Barcoding Kit (10 barcodes, 10 rxns) $50.00

$50.00 Add to cart

16250  Ion Set B Barcoding Kit (10 barcodes, 50 rxns) $250.00

$250.00 Add to cart