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Accel-NGS® 2S Indexed Adapters

New Indexed Adapters for Ultra-High-Throughput Multiplexed Sequencing

Get Better Sequencing Accuracy and Quadruple Your Throughput

The Accel-NGS 2S Indexed Adapters are a portfolio of indices designed to significantly increase NGS multiplexed sequencing on the Illumina® platform.

The Accel-NGS 2S Indexed Adapters are a unique set of 96 single indices optimized to work in combination with the Illumina TruSeq® HT P5 adapter to provide up to 768 unique dual index combinations.  The indices provide greater flexibility to process between 96-768 uniquely tagged samples per run and can accommodate a wider range of projects with varying sequencing depth. Additionally, the unique design eliminates misassignment and index hopping, an issue specific to Illumina’s pattern flow cells. All combined, improvements provide greater sensitivity to detect low frequency variants and greater fidelity to accelerate de-multiplexing during data analysis.

This industry-leading approach meets the growing demand from research laboratories and core laboratories for substantially higher throughput, greater flexibility with sample pooling options, and better data accuracy.

Features:

  • Automated sequencing of 96-768 samples per flow cell
  • Expands combinations for sample pooling options
  • Higher multiplexed pooling
  • Eliminates misassignments (< 0.1%) and index hopping
  • Inputs as low as 10 pg

Benefits:

  • 4x higher throughput
  • Greater flexibility in sample pooling
  • Lower labor and reagent cost per sample
  • More accurate de-multiplexing
  • Reduced concerns of proper quantification

The Accel-NGS 2S workflow is a standardized, sensitive protocol that requires as little as 10 pg of input DNA. This workflow incorporates improved purification steps to avoid index hopping.

Workflow for Accel-NGS 2S Single Indexed Adapters

The Accel-NGS 2S Single Indexed Adapters are fully compatible with the standard protocol and require no assay modification to incorporate the indices. This workflow contains one indexing step. During the Ligation I step, Reagent Y2 (a unique full-length Swift indexed adapter) is used to label each library. During the Ligation II step, Reagent B2 (the non-indexed P5 universal adapter) is ligated on the DNA library molecule. To sequence, a sample sheet is created by modifying the TruSeq LT template and saved as a .CSV file.  Using standard pipeline analyses, each index is then associated with a particular read-pair, identifying samples for downstream analysis.

Workflow for Accel-NGS 2S Dual Indexed Adapters

The Accel-NGS 2S Dual Indexed Adapters are full-length Swift indexed P7 and TruSeq HT P5 adapters. This workflow contains two indexing steps. During the Ligation I step, Reagent Y2 (a unique full-length Swift indexed adapater) is used to label each library. During the Ligation II step, Reagent B2 (a full-length TruSeq HT P5 adapter) is used to provide unique dual indexed combinations. To sequence, a sample sheet is created by modifying the TruSeq HT template and saved as a .CSV file.  When performing dual indexing, the i5 indexed adapter sequence (or its reverse compliment) is included depending on the sequence of choice. Using standard pipeline analyses, each Swift index, as well as the D50X index, are then associated with a particular read-pair, identifying samples for downstream analysis.

Place Order

An Indexed Adapter Kit is required for complete functionality with your Library Kit. Required Accel-NGS 2S Library Kits are sold separately.

Catalog No. Description Price QTY
26596 2S Set S1 Indexed Adapter Kit (24 indices, 96 rxns) $480.00

$480.00 Add to cart

26696 2S Set S2 Indexed Adapter Kit (24 indices, 96 rxns) $480.00

$480.00 Add to cart

26796 2S Set S3 Indexed Adapter Kit (24 indices, 96 rxns) $480.00

$480.00 Add to cart

26896 2S Set S4 Indexed Adapter Kit (24 indices, 96 rxns) $480.00

$480.00 Add to cart

269384 2S Set S1-S4 Indexed Adapter Kit (96 indices, 384 rxns) $1920.00

$1,920.00 Add to cart

27596 2S Set S1 MID Indexed Adapter Kit (24 indices, 96 rxns) $480.00

$480.00 Add to cart

27696 2S Set S2 MID Indexed Adapter Kit (24 indices, 96 rxns) $480.00

$480.00 Add to cart

27796 2S Set S3 MID Indexed Adapter Kit (24 indices, 96 rxns) $480.00

$480.00 Add to cart

27896 2S Set S4 MID Indexed Adapter Kit (24 indices, 96 rxns) $480.00

$480.00 Add to cart

279384 2S Set S1-S4 MID Indexed Adapter Kit (96 indices, 384 rxns) $1920.00

$1,920.00 Add to cart

28596 2S Set S1 Dual Indexed Adapter Kit (192 unique combinations) $1920.00

$1,920.00 Add to cart

28696 2S Set S2 Dual Indexed Adapter Kit (192 unique combinations) $1920.00

$1,920.00 Add to cart

28796 2S Set S3 Dual Indexed Adapter Kit (192 unique combinations) $1920.00

$1,920.00 Add to cart

28896 2S Set S4 Dual Indexed Adapter Kit (192 unique combinations) $1920.00

$1,920.00 Add to cart

289384 2S Set S1-S4 Dual Indexed Adapter Kit (768 unique combinations) $7680.00

$7,680.00 Add to cart