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Accel-NGS® Adaptase™ Module

Prepare Single-Cell Methyl-Seq Libraries

The Accel-NGS Adaptase Module constructs NGS libraries from bisulfite-converted, single-stranded DNA from single cells. Because bisulfite treatment results in lower quantity and quality DNA, the module is optimized to maximize the recovery of DNA containing uracil residues and low concentrations of AT-rich template. The resultant libraries consistently exhibit superior complexity with reduced composition bias to provide a more faithful representation of the methylome.

The Accel-NGS Adaptase Module is an excellent choice for single cell methylation applications. This approach incorporates a three-dimensional indexing strategy to generate 96 individual libraries that can be pooled and sequenced up to 384 individual cells in parallel. The published results demonstrated greater than two-fold increase in read mapping rate as compared to other methods and significantly improves the data output per run while reducing the sample sequencing cost.

It can be used for many applications, such as cataloging cell populations within heterogeneous tissues, assessing normal tissue for regulation of cellular mechanisms (e.g., differentiation), gaining insight into epigenetic alterations in disease states, and exploring across species to identify evolutionary conservation of epigenomic regulation.

Features:

  • Increased (> 2-fold) mapping rate
  • Larger (> 2-fold) library insert size
  • Workflow uses a single random priming cycle
  • Three-dimensional indexing for high-throughput sequencing

Benefits:

  • Greater data output per run and reduced sequencing cost
  • Efficient use of sequencing reads and greater data output per sequencing cluster
  • Easier low bias workflow
  • 384-plex single-cell sequencing for large volume sequencing instruments

Following lysis and bisulfite conversion of single cells, random priming of bisulfite-converted DNA incorporates a truncated P5 adapter (Truncated Adapter 1) to 5’ ends of synthesized fragments, and also effectively reduces fragment size to ~400 bp for suitability with Illumina® sequencing. Optionally, indexed random primers can be used for downstream multiplexing capability. Unused reagents are digested followed by a purification step. Next, the highly efficient Adaptase step simultaneously tails and ligates the truncated P7 adapter (Truncated Adapter 2) to the 3’ end of single-stranded products, which completes library construction. Library amplification is then performed using custom primers that incorporate dual indexing.

Place Order

First, select a product and quantity:

Catalog No. Description Price QTY
33096 Accel-NGS Adaptase Module (96 rxns) $4800

$4,800.00Add to cart

The below solution is included with your Library Kit. If you require extra, please add now:

Catalog No. Description Price QTY
90296  Low EDTA TE (96 rxns) $20.00

$20.00Add to cart