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Accel-NGS® XL Library Kit for Pacific Biosciences®

Reliable Long-Read Sequencing Solutions for Genome Assembly and Genomic Phasing

Get Better Quality Genome Assemblies. Faster.

The Swift Biosciences Accel-NGS XL Library Kit designed for the Pacific Biosciences (PacBio®) platform is the fastest sequencing solution for genome assembly and haplotype sequencing on Pacific Biosciences platforms. Long-read sequencing technology provides better coverage to sequence difficult genomic regions including low complexity, repetitive elements, or large structural variation. Until now, the standard protocol has had a long workflow that reduces your productivity.

The Accel-NGS XL Library Kit for PacBio is a novel library preparation kit optimized for Pacific Biosciences’ Single Molecule, Real-Time (SMRT®) sequencing. It provides significantly longer read lengths from less sample input along with a simple, single-tube workflow to accelerate your path to discovery. At the core, this library prep kit leverages Swift’s enhanced repair and ligation chemistry to produce a high library conversion rate, while simultaneously preventing adapter dimer formation and preserving DNA integrity.

This kit replaces the PacBio SMRTbell™ Template Prep Kit and works in conjunction with the PacBio DNA Polymerase Binding and MagBead loading kits. It is compatible with the PacBio RS II and Sequel™ platforms and contains sufficient material for constructing 16 sequencing libraries.

Features:

  • Generates average read length up to 20 kb
  • Ready-to-sequence libraries in < 5 hours
  • Single-tube assay for whole genome sequencing
  • Inputs as low as 2 µg

Benefits:

  • Improve your genomic assembly with longer read lengths
  • Double your productivity with libraries generated in less than a single day
  • Reduce labor cost and error rates with less sample processing
  • Compatible with a wider range of sample input amounts

The Accel-NGS XL workflow provides a simple, single-tube protocol consisting of  a 90-minute repair stage followed by a 30-minute ligation step, yielding a 4-hour procedure.

This single-tube workflow includes 4 incubation steps for internal and end repair, and ligates adapter sequences to the ends of fragmented dsDNA.

For more information, please contact Tech Support.

Protocols and Tools

  • Accel-NGS XL Library Prep Kit—coming soon

Scientific Posters

Place Order

First, select a product and quantity:

Catalog No. Description Price QTY
71016 Accel-NGS XL Library Kit (16 rxns) $2800.00

$2,800.00 Add to cart