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NEW! Swift 2S Turbo DNA Library Kits

The easy-to-use library prep kits for sequencing production labs

The NEW Swift 2S Turbo DNA Library Kits are a fast, efficient and cost-effective library prep solution.  Leveraging a robust enzymatic fragmentation prep and flexible indexing, you can prepare high-quality whole genome and exome libraries using a broad range of input amounts and sample types.

Swift 2S Turbo DNA Library Kits come in two configurations:

2S Turbo: All-in-one for quick implementation

Whether discovering or screening germline mutations, 2S Turbo makes library prep more accessible to any laboratory.  This kit configuration includes library prep reagents and Swift adapter compatible with several indexing options such a Single, Combinatorial Dual (up to 768-plex) or Unique Dual Indexing.

2S Turbo Flexible:  Broaden the range.  Expand your scale. 

Need a powerful pipeline to detect any variant across any sample quality and quantity? Combine our 2S Turbo Flexible with your choice of full-length indexed adapters (e.g. Illumina TruSeq DNA Single Indexes and TruSeq DNA CD, etc..) to create a fast, efficient, and scalable workflow to detect both rare and common variants in any size project.

2S Turbo with Swift Adapters

 

 

2S Turbo Flexible Workflow  (Use Adapter of Choice)

 

The Swift 2S Turbo Kits can be applied to:

  • Whole genome sequencing (WGS)
  • Metagenomic sequencing
  • Long-range PCR amplicons
  • RNA-Seq starting with full length, double-stranded cDNA input
  • Low-frequency somatic variation detection of SNVs and Indels
  • Copy number variation detection
  • Detection of germline inherited SNVs and Indels
  • Hybridization capture of relevant genomic regions (i.e., exome) or transcripts of interest

For indexing by PCR, we recommend using Swift’s truncated adapters in conjunction with dual (Combinatorial Dual Index Kits) and single (Indexing Primer Set A) indexed adapters, as well as, Turbo SureSelect Compatibility (for Agilent SureSelect capture). For optional PCR, we recommend using Illumina TruSeq DNA Single Indexes and TruSeq DNA CD (combinatorial dual) that are of similar design and are compatible with TA-ligation of dsDNA.

This protocol has been validated for library construction from 1 ng – 250 ng of double-stranded DNA. Please refer to the table below for recommended inputs of different types of DNA, for different sequencing applications.

  • Enzymatic prep, which consists of fragmentation of double-stranded DNA, followed by end-repair and dA-tailing of the fragments, all performed in a single reaction.
  • Adapter ligation, during which ds adapters are ligated to 3’-dA-tailed molecules.
  • Library amplification (optional), which employs high-fidelity, low-bias PCR to amplify libraries carrying adapter sequences on both ends. For lower inputs and certain indexing applications, we also offer indexing by PCR, during which truncated ds adapters are amplified to be rendered indexed and full-length.

This kit is validated for human genome as well as bacterial strains. However, standard fragmentation parameters may result in over-fragmentation of other low complexity samples, such as small viral genomes, long amplicons, and cDNA. For these sample types, the fragmentation time should be adjusted to achieve the desired mode fragment length. This makes control over the reaction time difficult, particularly when a large number of samples are processed manually. To enable more robust and reproductive results, the fragmentation temperature may be decreased to reduce enzymatic activity, thus increasing the time needed to achieve the desired fragment length.

It is possible to remove aliquots of the fragmentation reaction product for analysis. Fragmentation profiles may be informative when high sensitivity assays are used. The size distribution of fragments is expected to be approximately 350 bp and 200 bp for WGS and hybridization capture applications, respectively. The final size distribution of libraries prepared from FFPE samples is often smaller than expected based on the size distribution after fragmentation and adapter length. This phenomenon may be attributable to the inability of high fidelity DNA polymerase used in library amplification to efficiently amplify damaged DNA. However, when sequenced for target capture, a smaller insert size does not affect the % on-target.

The standard Swift protocol does not include size selection. However, if required, any commonly used size selection technique (e.g., the double-sided or an electrophoresis-based method) may be integrated into this protocol. Size selection should be carried out after the post-ligation clean-up or after library amplification.

Depending on the amount of library required for your application, it may be possible to omit library amplification. In such cases, it is important to ensure that your adapters are designed to support sample indexing and sequencing. The high conversion efficiency achievable with 2S Turbo enables PCR-free sequencing form as little as 50 ng of input DNA. Expect a greater than 20% DNA loss while performing the clean-up steps if PCR-free sequencing is desired.

For direct sequencing of libraries prepared from DNA inputs < 25 ng, adapter dilution is necessary to achieve low levels of adapter dimers. Adapter titration recommendations are outlined in the table below. Therefore, care quantification of your sample input is necessary for achieving optimal results.

Quantification of adapted libraries prior to amplification can greatly facilitate the optimization of library amplification parameters, particularly when a library construction workflow is first established or optimized for a diverse set of samples. Below are recommended minimum PCR cycles for direct sequencing of libraries prepared from high-quality genomic DNA. For samples of compromised quality, additional cycles may be required.

Over-amplification of library leads to an abnormal migration. During over-amplification, when DNA synthesis can no longer take place due to substrate depletion, subsequent rounds of DNA denaturation and annealing result in the formation of improperly annealed, partially double-stranded DNA, called heteroduplex structures that run larger than expected on the tape station.

 

  • Under digestion is primarily the reason you experience improper fragmentation. For optimal fragmentation, ensure your DNA is stored or eluted in a buffered solution containing Low EDTA TE (0.1 mM), as provided in this kit, to maximize fragmentation efficiency. If your DNA is in a 1 mM EDTA TE elution buffer used in the final steps of the DNA extraction or purification process, a buffer exchange using a column or bead-based purification step is required. If concerned about DNA loss. You can alternatively adjust the amount of Reagent K2 used in the Enzymatic Prep step to no more than 3x to achieve the desired fragment length.
  • Under digestion could also be the result of improper mixing of reagents. Ensure fragmentation master mix is adequately mixed prior to and after adding to the DNA samples.
  • Over digestion can occur if reaction left at room temperature. Ensure the Enzymatic Prep master mix and the DNA sample are kept on ice until placed onto the pre-chilled thermocycler.
  • Over digestion can also occur if sample integrity is compromised. Fragmentation time must be optimized for DNA samples that are not high molecular weight (e.g, FFPE). We have observed more rapid digestion for FFPE samples.

It is possible to remove aliquots of the fragmentation reaction product for analysis. Fragmentation profiles may be informative when high sensitivity assays are used. The size distribution of fragments is expected to be approximately 350 bp and 200 bp for WGS and hybridization capture applications, respectively. The final size distribution of libraries prepared from FFPE samples is often smaller than expected based on the size distribution after fragmentation and adapter length. This phenomenon may be attributable to the inability of high fidelity DNA polymerase used in library amplification to efficiently amplify damaged DNA. However, when sequenced for target capture, a smaller insert size does not affect the % on-target.

Place Order

First, select a product and quantity:

Catalog No. Description Price QTY
44024 Swift 2S Turbo DNA Library Kit (24 rxns) $360.00

$360.00Add to cart

44096 Swift 2S Turbo DNA Library Kit (96 rxns) $1440.00

$1,440.00Add to cart

45024 Swift 2S Turbo Flexible DNA Library Kit (24 rxns) $360.00

$360.00Add to cart

45096 Swift 2S Turbo Flexible DNA Library Kit (96 rxns) $1440.00

$1,440.00Add to cart

An Indexing Kit is required for complete functionality.
For the Swift 2S Turbo Flexible Kit, we recommend pairing with full length indexed adapters of your choice (e.g. Illumina TruSeq DNA Single Indexes and TruSeq DNA CD, etc...) that are of similar design and are compatible with TA-ligation of dsDNA.

Catalog No. Description Price QTY
46024  Swift 2S Turbo Single Indexing Primer Kit Set A (24 rxns) $120.00

$120.00Add to cart

48096  Swift 2S Turbo Combinatorial Dual Indexing Primer Kit (96 rxns) $480.00

$480.00Add to cart

49096  Swift 2S Turbo Unique Dual Indexing Kit (24 indices, 96 rxns) $480.00

$480.00Add to cart

490384  Swift 2S Turbo Unique Dual Indexing Kit (96 indices, 384 rxns) $1920.00

$1,920.00Add to cart

485192  Swift 2S Turbo Set S1 Combinatorial Dual Indexing Primer Kit (24x8 rxns) $1920.00

$1,920.00Add to cart

486192  Swift 2S Turbo Set S2 Combinatorial Dual Indexing Primer Kit (24x8 rxns) $1920.00

$1,920.00Add to cart

487192  Swift 2S Turbo Set S3 Combinatorial Dual Indexing Primer Kit (24x8 rxns) $1920.00

$1,920.00Add to cart

488192  Swift 2S Turbo Set S4 Combinatorial Dual Indexing Primer Kit (24x8 rxns) $1920.00

$1,920.00Add to cart

489768  Swift 2S Turbo Set S1-S4 Combinatorial Dual Indexing Primer Kit (96x8 rxns) $7680.00

$7,680.00Add to cart

46424  Swift 2S Turbo SureSelect Compatibility Module (24 rxns) $192.00

$192.00Add to cart

46496  Swift 2S Turbo SureSelect Compatibility Module (96 rxns) $768.00

$768.00Add to cart