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Accel-NGS® Unique Dual Indexing Kit

Boost Your Confidence with Less Errors

Swift’s Accel-NGS Unique Dual Indexing Kit (UDI) provides more reliable detection of low-frequency variants with significantly higher throughput and accurate demultiplexing during data analysis. For Unique Dual Indexing (UDI), each i7 and i5 index is used only once. This varies from standard combinatorial dual indexing where individual i5 and i7 indices are used multiple times in varied combinations. As a result, UDIs mitigate artifacts, such as index hopping and PCR-induced chimerism.  Any reassigned index reads that do not match another sample will be sorted as undetermined reads instead of being misassigned to another sample.

Leveraging a distinct design strategy on the i7 and i5 positions, Swift’s Unique Dual Indices reduce the read misassignment introduced by numerous mechanisms, including demultiplexing errors, PCR-induced chimerism during multiplexed hybridization capture, and index hopping on patterned flow cells, to <0.1%. These major improvements provide a more scalable reliable workflow for variant discovery and tumor profiling of complex biological specimens, such as FFPE and liquid biopsies.

The new Accel-NGS Unique Dual Indexing Kits are specifically designed and optimized for any Illumina platform and can be used for a wide variety of kits and applications, including:

Whole genome sequencing:

Whole exome:

Epigenetic analysis:

Features:

  • Optimized across all Illumina® platforms
  • Up to 96 unique dual index combinations for high throughput sequencing runs
  • Designed and optimized for 2- and 4-channel Illumina sequencing instruments with patterned or non-patterned flow cells

Benefits:

  • Improved detection of low-frequency variants (<5%)
  • Better data quality on patterned flow cells
  • Increased throughput and reduced cost per sample

UDIs are incorporated into truncated libraries via dual indexing by PCR and can be used with the 1S, Methyl, 2S Hyb and 2S Plus product. There are no specific workflow recommendations for UDI usage with Accel-NGS 2S Plus and Hyb DNA Library Kits, Accel-NGS 1S Plus and Methyl-Seq DNA Library Kits. The only modification to the standard workflows is a second clean-up post-amplification to ensure optimal removal of carryover adapters. This modification only applies when sequencing libraries on patterned flow cells.

Swift UDIs significantly reduce read misassignment due to index hopping on patterned flow cells

Place Order

First, select a product and quantity:

Catalog No. Description Price
29096 Accel-NGS 2S Unique Dual Indexing Kit (24 indices, 96 rxns) Inquire Find a Distributor
290384 Accel-NGS 2S Unique Dual Indexing Kit (96 indices, 384 rxns) Inquire Find a Distributor
39096 Accel-NGS Methyl-Seq Unique Dual Indexing Kit (24 indices, 96 rxns) Inquire Find a Distributor
390384 Accel-NGS Methyl-Seq Unique Dual Indexing Kit (96 indices, 384 rxns) Inquire Find a Distributor
19096 Accel-NGS 1S Unique Dual Indexing Kit (24 indices, 96 rxns) Inquire Find a Distributor
190384 Accel-NGS 1S Unique Dual Indexing Kit (96 indices, 384 rxns) Inquire Find a Distributor

Next, a Library Kit is required for complete functionality. Please select from the following:

Catalog No. Description Price
30024  Accel-NGS Methyl-Seq DNA Library Kit (24 rxns) Inquire Find a Distributor
30096  Accel-NGS Methyl-Seq DNA Library Kit (96 rxns) Inquire Find a Distributor
10024  Accel-NGS 1S Plus DNA Library Kit (24 rxns) Inquire Find a Distributor
10096  Accel-NGS 1S Plus DNA Library Kit (96 rxns) Inquire Find a Distributor
21024  Accel-NGS 2S Plus DNA Library Kit (24 rxns) Inquire Find a Distributor
21096  Accel-NGS 2S Plus DNA Library Kit (96 rxns) Inquire Find a Distributor
23024  Accel-NGS 2S Hyb DNA Library Kit (24) Inquire Find a Distributor
23096  Accel-NGS 2S Hyb DNA Library Kit (96) Inquire Find a Distributor