Save Time with Direct Conversion of 1st Strand cDNA
Robust Mapping and Strandedness
The Swift Rapid RNA Library Kit provides competitive metrics for routine transcriptomics, including high mapping rates and maintenance of strandedness.
Universal Human Reference (UHR) Total RNA (Agilent 740000) was poly(A) enriched using the NEBNext poly(A) mRNA Magnetic Isolation Module (NEB E7490) before beingn processed through the Swift Rapid RNA Library Kit or process following the supplier’s recommendations. Libraries were sequenced on a MiniSeq with 2 x 75 bp paired-end reads. Fastq files were downsampled to 3.8 million reads before analysis using STAR (mapping rate) or rnaseqc (strandedness).
Indexing Options, Kit Configurations, and Automation Support for Any Scale
The Swift Rapid RNA Library Kit uses an Indexing PCR step to complete the fully indexed adapter sequences, using primers that anneal to the truncated adapters added during the Random Priming and Adaptase steps, to be fully compatible with Illumina sequencers. Swift supplies a variety of index configurations and strategies, including:
- Single indexing
- Dual indexing as either:
- Combinatorial dual indexing, up to 768 combinations
- Unique dual indexing, up to 96 unique dual indices
Available in 24 or 96 reaction kit sizes, the Swift Rapid RNA Library Kit is offered in package sizes to support evaluation and adoption scales.
In addition, the Swift Rapid RNA Library Kit protocol is readily automatable. A 10% overage volume of reagents is supplied to accommodate automation. Please contact our automation team at [email protected] if you require additional reagent overage volume, if you would like to learn about our custom packaging options, or if you would like to request the assistance of our field support to develop and qualify optimized automated scripts with liquid handling platforms routinely used in NGS library preparation.
Please see ordering information below for kit formats, pricing and quantities.
First, select a product and quantity:
Swift Normalase is compatible with this kit. Please order both of the following.
For Customers Outside the United States: Please request a quote or select from our list of distributors.