Reproducible Outputs with Minimal Dimers and No Adapter Titration
Swift’s Adaptase technology allows for minimal adapter dimers with NO need for adapter titration. Compared to leading RNA library kits which can produce libraries with > 10% adapter dimers and require cumbersome adapter titration steps, the Swift RNA Library Kit produces <1% adapter dimers and maintains ligation efficiency at all supported input levels.
Libraries were prepared using both kits from the same quantity of input material and subjected to the same number of cycles of library amplification. Representative library sizes, yields, and Bioanalyzer traces illustrate typical libraries generated from the same input series of poly(A)-enriched Universal Human Reference (UHR) RNA or human brain mRNA when processed by either the Swift RNA Library Kit (left panel) or a leading RNA library kit (right panel). The arrows indicate adapter dimer peaks that were generated during library preparation.
High Mapping and Detection with Low Duplication Rates at Low Inputs
Universal Human Reference Total RNA (Agilent 740000) was poly(A) enriched using the NEBNext poly(A) mRNA Magnetic Isolation Module (NEB E7490) before being processed through the Swift RNA Library Kit or processed following the supplier recommendations. Libraries were sequenced on a MiniSeq with 2 x 75 bp paired-end reads. Fastq files were downsampled to 2.2 million reads before analysis using STAR (mapping rate), rnaseqc (genes/trancsripts detected), or picard (duplication rate).
Indexing Options, Kit Configurations, and Automation Support for Any Scale
The Swift RNA Library Kit leverages an Indexing PCR step to complete the fully indexed adapter sequences, using primers that anneal to the truncated adapters added during the Adaptase and Ligation steps, to be fully compatible with Illumina sequencers. Swift supplies a variety of index configurations and strategies, including:
- Single indexing
- Dual indexing as either:
- Combinatorial dual indexing, up to 768 combinations
- Unique dual indexing, up to 96 unique dual indices
Available in 24- or 96-reaction kit sizes, the Swift RNA Library Kit is offered at scales to support evaluation and adoption.
In addition, the Swift RNA Library Kit protocol is readily automatable. A 10% overage volume of reagents is supplied to accommodate automation. Please contact our automation team at [email protected] if you require additional reagent overage volume, if you would like to learn about our custom packaging options, or if you would like to request the assistance of our field support to develop and qualify optimized automated scripts with liquid handling platforms routinely used in NGS library preparation.
Please see ordering information below for kit formats, pricing and quantities.
First, select a product and quantity:
Swift Normalase is compatible with this kit. Please order both of the following.
For Customers Outside the United States: Please request a quote or select from our list of distributors.